Hotspot

atpF-atpH

LSC · 13858-14231 · 374 bp

Back to Sambucus

Pi 0.0155

MarkerSeek score 63.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 17
pi: 0.0155
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	57.9	58.2	452-491 bp	1.0	81.7
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	453-492 bp	1.0	81.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	483-522 bp	1.0	81.0
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	TGGCCTGGTTGTAGCATTAG	45.0	50.0	57.9	57.6	454-493 bp	1.0	80.3
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	CAGAGGGCAAAATACGAGGT	45.0	50.0	57.9	57.6	518-557 bp	1.0	80.1