Hotspot

trnH-GUG-psbA

LSC · 99-493 · 395 bp

Back to Sambucus

Pi 0.0163

MarkerSeek score 60.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 20
pi: 0.0163
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	464-635 bp	1.0	85.5
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	465-636 bp	1.0	85.2
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	457-628 bp	1.0	83.9
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	513-684 bp	1.0	83.1
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	514-685 bp	1.0	82.9