Hotspot

tRNA-Pro-tRNA-Leu

SSC · 109415-109631 · 217 bp

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Pi 0.156

MarkerSeek score 83.10

Primer available yes

Label tRNA-Pro-tRNA-Leu

Feature Metadata

label_name: tRNA-Pro-tRNA-Leu
feature_type: igs
region: SSC
length_bp: 217
variable_sites: 32
pi: 0.156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Pro-tRNA-Leu_p1	CGCTACCAAACTGCGCTATA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	276-281 bp	1.0	89.2
tRNA-Pro-tRNA-Leu_p2	CGCTACCAAACTGCGCTATA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.2	57.8	269-274 bp	1.0	88.8
tRNA-Pro-tRNA-Leu_p3	CGCTACCAAACTGCGCTATA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.2	57.8	275-280 bp	1.0	88.7
tRNA-Pro-tRNA-Leu_p4	CGCTACCAAACTGCGCTATA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.2	57.8	274-279 bp	1.0	88.7
tRNA-Pro-tRNA-Leu_p5	CGCTACCAAACTGCGCTATA	TAGGGCACTGCTTCCTAAGA	50.0	50.0	58.2	57.8	288-293 bp	1.0	88.1