Hotspot

tRNA-Asn-tRNA-Pro

SSC · 105007-109340 · 4334 bp

Back to Salvinia

Pi 0.109

MarkerSeek score 80.40

Primer available yes

Label tRNA-Asn-tRNA-Pro

Feature Metadata

label_name: tRNA-Asn-tRNA-Pro
feature_type: igs
region: SSC
length_bp: 4334
variable_sites: 467
pi: 0.109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn-tRNA-Pro_p1	TCATTCTCGTTTGTTTGCGC	AGTTACGTGCGGTATGGAAC	45.0	50.0	58.0	58.0	672 bp	0.5	59.7
tRNA-Asn-tRNA-Pro_p2	GAAACGTACTTCGGGTTCGA	AGTTACGTGCGGTATGGAAC	50.0	50.0	57.9	58.0	534 bp	0.5	59.6
tRNA-Asn-tRNA-Pro_p3	AGAAACGTACTTCGGGTTCG	AGTTACGTGCGGTATGGAAC	50.0	50.0	57.9	58.0	535 bp	0.5	59.5
tRNA-Asn-tRNA-Pro_p4	CTCAGCCCGGTCCATTTTAA	AGTTACGTGCGGTATGGAAC	50.0	50.0	57.9	58.0	450 bp	0.5	59.0
tRNA-Asn-tRNA-Pro_p5	TCAGCCCGGTCCATTTTAAG	AGTTACGTGCGGTATGGAAC	50.0	50.0	57.9	58.0	449 bp	0.5	59.0