Hotspot

tRNA-Ile-tRNA-Arg

IRb · 84201-84986 · 786 bp

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Pi 0.0687
MarkerSeek score 84.10
Primer available yes
Label tRNA-Ile-tRNA-Arg

Feature Metadata

label_name
tRNA-Ile-tRNA-Arg
feature_type
igs
region
IRb
length_bp
786
variable_sites
50
pi
0.0687
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Ile-tRNA-Arg_p1 TGAACCTGTGAATTCGCCAA CAGAGGATTAGAGCACGTGG 45.0 55.0 58.0 57.8 905 bp 0.5 58.1
tRNA-Ile-tRNA-Arg_p2 TTGAACCTGTGAATTCGCCA CAGAGGATTAGAGCACGTGG 45.0 55.0 58.0 57.8 906 bp 0.5 58.1
tRNA-Ile-tRNA-Arg_p3 TAACCGTTCAGCCATGGATG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.0 57.8 868 bp 0.5 58.0
tRNA-Ile-tRNA-Arg_p4 TTGGGTGCTTTAACCGTTCA CAGAGGATTAGAGCACGTGG 45.0 55.0 57.9 57.8 878 bp 0.5 57.8
tRNA-Ile-tRNA-Arg_p5 AGTTGGGTGCTTTAACCGTT CAGAGGATTAGAGCACGTGG 45.0 55.0 57.9 57.8 880 bp 0.5 57.8