Hotspot

tRNA-Cys-tRNA-Gly

LSC · 30868-31222 · 355 bp

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Pi 0.159

MarkerSeek score 79.80

Primer available yes

Label tRNA-Cys-tRNA-Gly

Feature Metadata

label_name: tRNA-Cys-tRNA-Gly
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 52
pi: 0.159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Cys-tRNA-Gly_p1	CAGATTCGAACTGGGGGATG	GTTCGATTCCCGCTATCCG	55.0	57.9	58.0	58.2	430-438 bp	1.0	82.2
tRNA-Cys-tRNA-Gly_p2	CAGATTCGAACTGGGGGATG	GGGTTCGATTCCCGCTATC	55.0	57.9	58.0	57.8	432-440 bp	1.0	82.0
tRNA-Cys-tRNA-Gly_p3	CAGATTCGAACTGGGGGATG	GGTTCGATTCCCGCTATCC	55.0	57.9	58.0	57.8	431-439 bp	1.0	82.0
tRNA-Cys-tRNA-Gly_p4	AGATTCGAACTGGGGGATGA	GTTCGATTCCCGCTATCCG	50.0	57.9	58.1	58.2	429-437 bp	1.0	81.5
tRNA-Cys-tRNA-Gly_p5	GGGGGATGAAGGATTTGCAA	GTTCGATTCCCGCTATCCG	50.0	57.9	58.1	58.2	418-426 bp	1.0	81.4