Hotspot

tRNA-Glu-tRNA-Cys

LSC · 29234-30795 · 1562 bp

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Pi 0.124

MarkerSeek score 86.20

Primer available yes

Label tRNA-Glu-tRNA-Cys

Feature Metadata

label_name: tRNA-Glu-tRNA-Cys
feature_type: igs
region: LSC
length_bp: 1562
variable_sites: 180
pi: 0.124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu-tRNA-Cys_p1	TTGTCCTAGGCCTCTAGACG	CATCCCCCAGTTCGAATCTG	55.0	55.0	57.9	58.0	1541-1620 bp	1.0	91.2
tRNA-Glu-tRNA-Cys_p2	TTGTCCTAGGCCTCTAGACG	TCATCCCCCAGTTCGAATCT	55.0	50.0	57.9	58.1	1542-1621 bp	1.0	90.6
tRNA-Glu-tRNA-Cys_p3	TTGTCCTAGGCCTCTAGACG	TTGCAAATCCTTCATCCCCC	55.0	50.0	57.9	58.1	1553-1632 bp	1.0	90.5
tRNA-Glu-tRNA-Cys_p4	AGAGAGTTGTCCTAGGCCTC	CATCCCCCAGTTCGAATCTG	55.0	55.0	57.8	58.0	1547-1626 bp	1.0	90.4
tRNA-Glu-tRNA-Cys_p5	GAGAGTTGTCCTAGGCCTCT	CATCCCCCAGTTCGAATCTG	55.0	55.0	57.8	58.0	1546-1625 bp	1.0	90.4