Hotspot

tRNA-Asp-tRNA-Tyr

LSC · 28787-28902 · 116 bp

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Pi 0.196
MarkerSeek score 81.00
Primer available yes
Label tRNA-Asp-tRNA-Tyr

Feature Metadata

label_name
tRNA-Asp-tRNA-Tyr
feature_type
igs
region
LSC
length_bp
116
variable_sites
20
pi
0.196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Asp-tRNA-Tyr_p1 TCAATTGGTTAGAGCACCGC CTCGAGTGGTTAATGGGGAC 50.0 55.0 58.6 57.7 238-252 bp 1.0 83.6
tRNA-Asp-tRNA-Tyr_p2 TCAATTGGTTAGAGCACCGC ATGCTCGAGTGGTTAATGGG 50.0 50.0 58.6 57.7 241-255 bp 1.0 83.4
tRNA-Asp-tRNA-Tyr_p3 GGTTCGAGACCCGTCAATC CTCGAGTGGTTAATGGGGAC 57.9 55.0 58.0 57.7 197-211 bp 1.0 81.0
tRNA-Asp-tRNA-Tyr_p4 GTTCGAGACCCGTCAATCC CTCGAGTGGTTAATGGGGAC 57.9 55.0 58.0 57.7 196-210 bp 1.0 81.0
tRNA-Asp-tRNA-Tyr_p5 GTTCGAGACCCGTCAATCC ATGCTCGAGTGGTTAATGGG 57.9 50.0 58.0 57.7 199-213 bp 1.0 80.7