Hotspot

tRNA-Gly-tRNA-Arg

LSC · 11064-11207 · 144 bp

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Pi 0.2
MarkerSeek score 78.90
Primer available yes
Label tRNA-Gly-tRNA-Arg

Feature Metadata

label_name
tRNA-Gly-tRNA-Arg
feature_type
igs
region
LSC
length_bp
144
variable_sites
25
pi
0.2
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Gly-tRNA-Arg_p1 ACCCTTAGCCTTCCAAGCTA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 230-233 bp 1.0 75.3
tRNA-Gly-tRNA-Arg_p2 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 236-239 bp 1.0 74.7
tRNA-Gly-tRNA-Arg_p3 TTCCAAGCTAATGATGCGGG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.3 56.9 220-223 bp 1.0 73.0
tRNA-Gly-tRNA-Arg_p4 TTCCAAGCTAATGATGCGGG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.3 57.9 226-229 bp 1.0 72.4
tRNA-Gly-tRNA-Arg_p5 GCTAATGATGCGGGTTCGAT AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.5 56.9 214-217 bp 1.0 71.7