Hotspot

tRNA-Gly-tRNA-Gly

LSC · 10177-11015 · 839 bp

Back to Salvinia

Pi 0.0846

MarkerSeek score 82.90

Primer available yes

Label tRNA-Gly-tRNA-Gly

Feature Metadata

label_name: tRNA-Gly-tRNA-Gly
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 69
pi: 0.0846
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gly-tRNA-Gly_p1	TTACTTTGTGGCGGGTATGG	CCCGCATCATTAGCTTGGAA	50.0	50.0	57.9	58.3	900-924 bp	1.0	84.6
tRNA-Gly-tRNA-Gly_p2	TTGTGGCGGGTATGGTTTAG	CCCGCATCATTAGCTTGGAA	50.0	50.0	57.9	58.3	895-919 bp	1.0	84.6
tRNA-Gly-tRNA-Gly_p3	TTACTTTGTGGCGGGTATGG	ATCGAACCCGCATCATTAGC	50.0	50.0	57.9	58.5	906-930 bp	1.0	83.2
tRNA-Gly-tRNA-Gly_p4	TTGTGGCGGGTATGGTTTAG	ATCGAACCCGCATCATTAGC	50.0	50.0	57.9	58.5	901-925 bp	1.0	83.2
tRNA-Gly-tRNA-Gly_p5	TTGTGGCGGGTATGGTTTAG	AACCCGCATCATTAGCTTGG	50.0	50.0	57.9	58.6	897-921 bp	1.0	82.3