Hotspot

ndhF

SSC · 107880-110111 · 2232 bp

Pi 0.0258

MarkerSeek score 66.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 425
pi: 0.0258
unique_haplotype_count: 92
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACGCGTTTCTGAAGAGTC	CTCGTTGGTATAGCGGGATG	50.0	55.0	57.9	57.9	2724-2730 bp	0.152381	47.7
ndhF_p2	GACGCGTTTCTGAAGAGTCT	CTCGTTGGTATAGCGGGATG	50.0	55.0	57.9	57.9	2723-2729 bp	0.152381	47.7
ndhF_p3	AAGAGGGATCCACCGAAGAA	CTCGTTGGTATAGCGGGATG	50.0	55.0	58.0	57.9	2873-2879 bp	0.152381	47.6
ndhF_p4	ACAGGAACAAGAGGGATCCA	CTCGTTGGTATAGCGGGATG	50.0	55.0	58.0	57.9	2881-2886 bp	0.142857	47.1
ndhF_p5	AAGGCGGATCCGAACAAAAT	CTCGTTGGTATAGCGGGATG	45.0	55.0	58.2	57.9	2823-2829 bp	0.152381	46.7