Hotspot

rps15-ycf1

SSC · 120569-120974 · 406 bp

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Pi 0.0573
MarkerSeek score 70.80
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
406
variable_sites
127
pi
0.0573
unique_haplotype_count
66
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGTCGTTGACGTTTTCCCAA TGGGACGCAACTTTGAATCA 45.0 45.0 58.0 58.0 1260-1342 bp 1.0 91.6
rps15-ycf1_p2 AGTCGTTGACGTTTTCCCAA CGTAACAAACAGCAGCCAAG 45.0 50.0 58.0 58.0 754-836 bp 0.942857 89.2
rps15-ycf1_p3 AGTCGTTGACGTTTTCCCAA AGCAGCCAAGTTTCACATGA 45.0 45.0 58.0 58.0 744-826 bp 0.92381 88.6
rps15-ycf1_p4 AACACCATGCATTGATGGGT AGCAGCCAAGTTTCACATGA 45.0 45.0 58.1 58.0 993-1085 bp 0.92381 88.4
rps15-ycf1_p5 AGTCGTTGACGTTTTCCCAA GAACGTAACAAACAGCAGCC 45.0 50.0 58.0 58.0 778-839 bp 0.352381 65.4