Hotspot

ccsA-ndhD

SSC · 112543-112779 · 237 bp

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Pi 0.071
MarkerSeek score 68.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
237
variable_sites
100
pi
0.071
unique_haplotype_count
63
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACATAGTTATGGTTCATTTACATT ACCCAGATTTCGTTCTTTCACT 25.0 40.9 52.5 57.9 258-360 bp 0.990476 51.6
ccsA-ndhD_p2 AGTTATGGTTCATTTACATTAACA ACCCAGATTTCGTTCTTTCACT 25.0 40.9 52.7 57.9 254-356 bp 0.980952 51.2
ccsA-ndhD_p3 ATGGTTCATTTACATTAACATCT ACCCAGATTTCGTTCTTTCACT 26.1 40.9 52.1 57.9 250-352 bp 0.952381 50.1
ccsA-ndhD_p4 AGTTATGGTTCATTTACATTAACATCT ACCCAGATTTCGTTCTTTCACT 25.9 40.9 55.7 57.9 254-356 bp 0.952381 50.1
ccsA-ndhD_p5 ATGGTTCATTTACATTAACATCT ACCCAGATTTCGTTCTTTCAC 26.1 42.9 52.1 56.5 250-352 bp 0.942857 49.7