Hotspot

ndhF-rpl32

SSC · 110112-110569 · 458 bp

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Pi 0.045
MarkerSeek score 73.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
458
variable_sites
134
pi
0.045
unique_haplotype_count
83
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTTGCAAAAGCCCACATACG CGTGAATTGATGGCGAGAGA 50.0 50.0 58.0 58.1 976-1124 bp 0.657143 77.5
ndhF-rpl32_p2 TGGTTGCTGTCGGAAAAAGA CGTGAATTGATGGCGAGAGA 45.0 50.0 58.0 58.1 945-1093 bp 0.609524 75.4
ndhF-rpl32_p3 ACACATTCCAACCAGTTCCC CGTGAATTGATGGCGAGAGA 50.0 50.0 58.0 58.1 1421-1464 bp 0.580952 74.6
ndhF-rpl32_p4 CACATTCCAACCAGTTCCCA CGTGAATTGATGGCGAGAGA 50.0 50.0 58.0 58.1 1420-1463 bp 0.580952 74.6
ndhF-rpl32_p5 AGTGGATCGATCAAGCAACC CGTGAATTGATGGCGAGAGA 50.0 50.0 58.0 58.1 1260-1268 bp 0.161905 50.0