Hotspot

petA-psbJ

LSC · 61729-62737 · 1009 bp

Back to Salvia

Pi 0.0318
MarkerSeek score 72.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1009
variable_sites
241
pi
0.0318
unique_haplotype_count
91
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TTTATCCCGACGGAAGCAAG GTCTCTAGCACGACCACTTG 50.0 55.0 58.0 58.0 1510-1629 bp 0.952381 89.8
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC GTCTCTAGCACGACCACTTG 50.0 55.0 58.0 58.0 1246-1365 bp 0.952381 89.7
petA-psbJ_p3 TCCGATCGTATTTCTCCCGA GTCTCTAGCACGACCACTTG 50.0 55.0 58.0 58.0 1730-1849 bp 0.914286 88.2
petA-psbJ_p4 TTTATCCCGACGGAAGCAAG TGCGGGTTGGTTATTTGTCA 50.0 45.0 58.0 58.0 2051-2118 bp 0.285714 56.2
petA-psbJ_p5 TCCGATCGTATTTCTCCCGA TGCGGGTTGGTTATTTGTCA 50.0 45.0 58.0 58.0 2271-2338 bp 0.285714 56.0