Hotspot

ndhC-trnV-UAC

LSC · 49479-50282 · 804 bp

Back to Salvia

Pi 0.027
MarkerSeek score 70.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
804
variable_sites
176
pi
0.027
unique_haplotype_count
76
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 27.3 50.0 52.7 57.8 859-1031 bp 1.0 52.0
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAAAAACA GAAGGTCTACGGTTCGAGTC 27.3 55.0 52.7 57.5 858-1030 bp 1.0 52.0
ndhC-trnV-UAC_p3 ATCATATTCGTAAAGCAAAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 860-1032 bp 1.0 52.0
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAAAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 53.3 57.8 859-1031 bp 1.0 52.0
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAAAAACA GTTCGAGTCCGTATAGCCCT 27.3 55.0 52.7 58.7 847-1019 bp 0.990476 51.6