Hotspot

trnS-GCU-trnG-UCC

LSC · 8059-8762 · 704 bp

Back to Salvia

Pi 0.0313
MarkerSeek score 70.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
704
variable_sites
152
pi
0.0313
unique_haplotype_count
79
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 659-2895 bp 1.0 59.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 658-2894 bp 1.0 59.9
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 660-2896 bp 1.0 58.8
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 659-2895 bp 1.0 58.8
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA TACCACTAAACTATACCCGC 50.0 45.0 58.2 54.0 713-2896 bp 1.0 58.7