Hotspot

psbK-psbI

LSC · 7320-7711 · 392 bp

Back to Salvia

Pi 0.043
MarkerSeek score 70.10
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
392
variable_sites
96
pi
0.043
unique_haplotype_count
65
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 TAGCTTTTGTTTGGCAGGCT ACGAAGAGTTTCAGAGTAAGCA 45.0 40.9 58.3 57.4 434-1855 bp 1.0 68.9
psbK-psbI_p2 GCAGGCTGCTGTAAGTTTTC ACGAAGAGTTTCAGAGTAAGCA 50.0 40.9 57.7 57.4 421-1856 bp 1.0 68.6
psbK-psbI_p3 TTAGCTTTTGTTTGGCAGGC ACGAAGAGTTTCAGAGTAAGCA 45.0 40.9 57.5 57.4 435-1853 bp 1.0 67.1
psbK-psbI_p4 AGGCTGCTGTAAGTTTTCGA ACGAAGAGTTTCAGAGTAAGCA 45.0 40.9 57.5 57.4 419-1854 bp 1.0 67.0
psbK-psbI_p5 CAGGCTGCTGTAAGTTTTCG ACGAAGAGTTTCAGAGTAAGCA 50.0 40.9 57.4 57.4 420-1855 bp 1.0 66.5