Hotspot

rps16-trnQ-UUG

LSC · 5919-6672 · 754 bp

Back to Salvia

Pi 0.0343
MarkerSeek score 70.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
754
variable_sites
194
pi
0.0343
unique_haplotype_count
83
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 262-1146 bp 1.0 90.3
rps16-trnQ-UUG_p2 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 254-1138 bp 1.0 85.1
rps16-trnQ-UUG_p3 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 261-1145 bp 1.0 84.5
rps16-trnQ-UUG_p4 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 257-1141 bp 1.0 82.9
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTCCC 45.0 57.9 58.1 57.9 261-1143 bp 1.0 82.4