Hotspot

trnK-UUU-rps16

LSC · 4250-4819 · 570 bp

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Pi 0.0413
MarkerSeek score 74.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
570
variable_sites
139
pi
0.0413
unique_haplotype_count
77
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTAGCCGCACTTAAAAGCC CTTTTAAAGAAGGCGGGAGT 50.0 45.0 58.3 55.7 627-655 bp 0.914286 67.7
trnK-UUU-rps16_p2 CCGCACTTAAAAGCCGAGTA CTTTTAAAGAAGGCGGGAGT 50.0 45.0 58.3 55.7 622-650 bp 0.914286 67.5
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC AGAAGGCGGGAGTTTTTAAA 50.0 40.0 58.3 55.2 487-648 bp 0.990476 66.8
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC CTTTTAAAGAAGGCGGGAGTT 50.0 42.9 58.3 56.4 627-655 bp 0.914286 65.9
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA CTTTTAAAGAAGGCGGGAGTT 50.0 42.9 58.3 56.4 622-650 bp 0.914286 65.8