Hotspot

trnH-GUG-psbA

LSC · 76-393 · 318 bp

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Pi 0.0636
MarkerSeek score 76.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
318
variable_sites
118
pi
0.0636
unique_haplotype_count
75
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 370-457 bp 1.0 89.6
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 318-405 bp 0.971429 89.2
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 332-419 bp 0.971429 89.2
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 331-418 bp 0.971429 89.2
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 333-420 bp 0.933333 87.7