Hotspot

ndhF-rpl32

SSC · 111842-112954 · 1113 bp

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Pi 0.0462
MarkerSeek score 77.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1113
variable_sites
129
pi
0.0462
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCAATAGGTCGTGTGAACCA AAAAGGCTTTTAACGCTGCC 50.0 45.0 58.2 57.9 1709-1738 bp 1.0 87.8
ndhF-rpl32_p2 GCCCATATACGCCGAAGATT AAAAGGCTTTTAACGCTGCC 50.0 45.0 57.9 57.9 1319-1348 bp 0.888889 84.3
ndhF-rpl32_p3 AAAGCCCATATACGCCGAAG AAAAGGCTTTTAACGCTGCC 50.0 45.0 58.1 57.9 1322-1351 bp 0.888889 84.0
ndhF-rpl32_p4 ATGAAGGGGAAATTGGGCAG AAAAGGCTTTTAACGCTGCC 50.0 45.0 58.1 57.9 1951-1967 bp 0.666667 65.0
ndhF-rpl32_p5 GAAGGGGAAATTGGGCAGAT AAAAGGCTTTTAACGCTGCC 50.0 45.0 57.8 57.9 1949-1965 bp 0.666667 64.7