Hotspot

psbE-petL

LSC · 64557-65703 · 1147 bp

Back to Salsola

Pi 0.0338

MarkerSeek score 71.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 103
pi: 0.0338
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAATAACTAGTTAGAGTAGGCAT	50.0	30.4	57.8	52.4	1193-1309 bp	1.0	50.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAATAACTAGTTAGAGTAGGCAT	50.0	30.4	57.9	52.4	1270-1386 bp	1.0	50.3
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAATAACTAGTTAGAGTAGGCAT	45.0	30.4	58.2	52.4	1279-1395 bp	1.0	50.2
psbE-petL_p4	TGCTGACGAATAACCAACCC	AAATAACTAGTTAGAGTAGGCAT	50.0	30.4	58.2	52.4	1284-1400 bp	1.0	50.2
psbE-petL_p5	CGAATAACCAACCCGCAATG	AAATAACTAGTTAGAGTAGGCAT	50.0	30.4	57.8	52.4	1278-1394 bp	1.0	50.2