Hotspot

trnF-GAA-ndhJ

LSC · 48134-48804 · 671 bp

Back to Salsola

Pi 0.0332

MarkerSeek score 70.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 60
pi: 0.0332
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTTATTGA	50.0	26.1	58.1	52.2	727-757 bp	1.0	49.5
trnF-GAA-ndhJ_p2	CTGGTAGAGCAGAGGACTGA	ATGAAATACAAGATGCTTATTGA	55.0	26.1	57.9	52.2	751-781 bp	1.0	49.2
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATGAAATACAAGATGCTTATTGA	55.0	26.1	58.0	52.2	763-793 bp	1.0	49.1
trnF-GAA-ndhJ_p4	AAGGGTGAATGAATGGTCGG	ATGAAATACAAGATGCTTATTGA	50.0	26.1	57.9	52.2	781-811 bp	1.0	48.9
trnF-GAA-ndhJ_p5	TGAGAAGATGATGCGCCAAA	ATGAAATACAAGATGCTTATTGA	45.0	26.1	57.9	52.2	800-834 bp	1.0	48.7