Hotspot

rps4-trnT-UGU

LSC · 45835-46268 · 434 bp

Back to Salsola

Pi 0.0527
MarkerSeek score 78.90
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
434
variable_sites
50
pi
0.0527
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 AGTCCAGGTAAAGCCCCTAG CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 535-567 bp 1.0 89.2
rps4-trnT-UGU_p2 GTCCAGGTAAAGCCCCTAGA CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 534-566 bp 1.0 89.2
rps4-trnT-UGU_p3 TCCAGGTAAAGCCCCTAGAC CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.1 57.9 533-565 bp 1.0 89.2
rps4-trnT-UGU_p4 AGTCCAGGTAAAGCCCCTAG ATGGTCATCGGTTCGATTCC 55.0 50.0 58.1 57.8 503-535 bp 1.0 88.8
rps4-trnT-UGU_p5 TCCAGGTAAAGCCCCTAGAC ATGGTCATCGGTTCGATTCC 55.0 50.0 58.1 57.8 501-533 bp 1.0 88.8