Hotspot

trnD-GUC-trnY-GUA

LSC · 29980-30382 · 403 bp

Back to Salsola

Pi 0.0494

MarkerSeek score 74.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 54
pi: 0.0494
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GCGGTGCTCTAACCAATTGA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	451-474 bp	1.0	85.7
trnD-GUC-trnY-GUA_p2	GCGGTGCTCTAACCAATTGA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.6	57.8	450-473 bp	1.0	85.7
trnD-GUC-trnY-GUA_p3	GCGGTGCTCTAACCAATTGA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.6	58.4	472-495 bp	1.0	84.8
trnD-GUC-trnY-GUA_p4	GCGGTGCTCTAACCAATTGA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.6	57.6	471-494 bp	1.0	84.6
trnD-GUC-trnY-GUA_p5	GCGGTGCTCTAACCAATTGA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.6	57.5	467-490 bp	1.0	83.4