Hotspot

rpoB-trnC-GCA

LSC · 26378-27523 · 1146 bp

Back to Salsola

Pi 0.0433

MarkerSeek score 71.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 126
pi: 0.0433
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CACGAGCCCATATTCTTGCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1684-1749 bp	1.0	89.0
rpoB-trnC-GCA_p2	GAAAATACCGGGTCTCCACC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1889-1954 bp	1.0	88.1
rpoB-trnC-GCA_p3	CCCGGGCTTTGCAATATTTG	ATAGGAATGGGGAGGTCTCG	50.0	55.0	58.1	58.0	1798-1857 bp	0.888889	84.2
rpoB-trnC-GCA_p4	CACGAGCCCATATTCTTGCT	ATAGGAATGGGGAGGTCTCG	50.0	55.0	58.0	58.0	1920-1979 bp	0.888889	83.9
rpoB-trnC-GCA_p5	GAAAATACCGGGTCTCCACC	ATAGGAATGGGGAGGTCTCG	55.0	55.0	58.0	58.0	2125-2184 bp	0.888889	83.2