Hotspot

rps16-trnQ-UUG

LSC · 5925-6482 · 558 bp

Back to Salsola

Pi 0.0556

MarkerSeek score 77.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 84
pi: 0.0556
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	628-669 bp	1.0	90.3
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	639-680 bp	1.0	89.8
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	658-699 bp	1.0	89.5
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.2	58.2	669-710 bp	1.0	89.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	45.0	50.0	58.1	58.5	659-700 bp	1.0	87.2