Hotspot

rps18

Genome · 89547-89969 · 423 bp

Back to Salmonopuntia

Pi 0.00709

MarkerSeek score 48.20

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 423
variable_sites: 3
pi: 0.00709
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CGTTTGTGGATCACTCGGAT	CTAGTCGGTTGGAATTGCGA	50.0	50.0	58.0	58.0	1271-1307 bp	1.0	81.7
rps18_p2	CGTTTGTGGATCACTCGGAT	AGGAGGTTAGGTTGATGGCT	50.0	50.0	58.0	58.0	1412-1448 bp	1.0	81.3
rps18_p3	CGTTTGTGGATCACTCGGAT	GGAGGTTAGGTTGATGGCTG	50.0	55.0	58.0	58.0	1411-1447 bp	1.0	81.2
rps18_p4	CGTTTGTGGATCACTCGGAT	GGTTAGGTTGATGGCTGAGG	50.0	55.0	58.0	58.0	1408-1444 bp	1.0	81.2
rps18_p5	CGTTTGTGGATCACTCGGAT	GTTAGGTTGATGGCTGAGGG	50.0	55.0	58.0	58.0	1407-1443 bp	1.0	81.2