Hotspot

rps12

Genome · 1152-1939 · 788 bp

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Pi 0.00761

MarkerSeek score 62.80

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: Genome
length_bp: 788
variable_sites: 6
pi: 0.00761
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	TACGGTTCTGTAGAGTGGCA	CAGTACCTCGACGTGACATG	50.0	55.0	58.1	58.1	909 bp	1.0	79.0
rps12_p2	TACGGTTCTGTAGAGTGGCA	TACAGTACCTCGACGTGACA	50.0	50.0	58.1	57.8	911 bp	1.0	78.5
rps12_p3	TACGGTTCTGTAGAGTGGCA	GTACCTCGACGTGACATGAG	50.0	55.0	58.1	57.8	907 bp	1.0	78.4
rps12_p4	TACGGTTCTGTAGAGTGGCA	AGTACCTCGACGTGACATGA	50.0	50.0	58.1	58.2	908 bp	1.0	78.4
rps12_p5	ATTGAACGAGGAGCCGTATG	CAGTACCTCGACGTGACATG	50.0	55.0	57.8	58.1	945 bp	1.0	77.9