Hotspot

rpl23

Genome · 12689-12955 · 267 bp

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Pi 0.0112

MarkerSeek score 44.30

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 267
variable_sites: 3
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	GAATGGTTAAAGCGCCCAAC	CGGTATAGACGCTTATGGCC	50.0	55.0	58.0	58.0	683 bp	1.0	80.4
rpl23_p2	GAATGGTTAAAGCGCCCAAC	CTTAGATCCGGCTCTACCCA	50.0	55.0	58.0	58.0	1131 bp	1.0	80.3
rpl23_p3	GAATGGTTAAAGCGCCCAAC	TTATGGCCTCCCCCTCTATG	50.0	55.0	58.0	58.0	671 bp	1.0	80.2
rpl23_p4	CCAAATTCGGGGGTCAAAGA	CTTAGATCCGGCTCTACCCA	50.0	55.0	58.1	58.0	1472-1474 bp	1.0	79.7
rpl23_p5	TCCAAATTCGGGGGTCAAAG	CTTAGATCCGGCTCTACCCA	50.0	55.0	58.1	58.0	1473-1475 bp	1.0	79.7