Hotspot

petA-psbJ

Genome · 84964-86004 · 1041 bp

Back to Salmonopuntia

Pi 0.00576

MarkerSeek score 62.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1041
variable_sites: 6
pi: 0.00576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATTGCGCGTACAAGGTCTTT	ACTGGCATTCTTGTGATCGT	45.0	45.0	58.5	57.5	1231-1234 bp	1.0	72.7
petA-psbJ_p2	ATTGCGCGTACAAGGTCTTT	CGTATTCTGGATTGGGCTCA	45.0	50.0	58.5	57.4	1185-1188 bp	1.0	71.6
petA-psbJ_p3	ATTGCGCGTACAAGGTCTTT	ATTCTGGATTGGGCTCATCC	45.0	50.0	58.5	57.3	1182-1185 bp	1.0	71.2
petA-psbJ_p4	TTGCGCGTACAAGGTCTTTT	ACTGGCATTCTTGTGATCGT	45.0	45.0	58.7	57.5	1230-1233 bp	1.0	71.0
petA-psbJ_p5	TTGCGCGTACAAGGTCTTTT	CGTATTCTGGATTGGGCTCA	45.0	50.0	58.7	57.4	1184-1187 bp	1.0	69.9