Hotspot

atpA-atpF

Genome · 29944-30012 · 69 bp

Back to Salmonopuntia

Pi 0.058

MarkerSeek score 82.20

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: Genome
length_bp: 69
variable_sites: 4
pi: 0.058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CAATGCCGTCACCTACTTGA	GCATGTTTGGTGCCATGAAA	50.0	45.0	57.9	57.8	222 bp	1.0	79.4
atpA-atpF_p2	ATGCCGTCACCTACTTGAAG	GCATGTTTGGTGCCATGAAA	50.0	45.0	57.6	57.8	220 bp	1.0	77.0
atpA-atpF_p3	TGCTAATTTCGTCTGCTCGA	GCATGTTTGGTGCCATGAAA	45.0	45.0	57.4	57.8	135 bp	1.0	75.9
atpA-atpF_p4	AATGCCGTCACCTACTTGAA	GCATGTTTGGTGCCATGAAA	45.0	45.0	57.1	57.8	221 bp	1.0	73.3
atpA-atpF_p5	TGCCGTCACCTACTTGAAGT	GCATGTTTGGTGCCATGAAA	50.0	45.0	59.0	57.8	219 bp	1.0	72.5