Hotspot

ndhF

SSC · 115782-118136 · 2355 bp

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Pi 0.00824
MarkerSeek score 49.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2355
variable_sites
102
pi
0.00824
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.96
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TGAAAACAAAGAGAGACGTCCA AGCGTGTCTACCGATTTCAC 40.9 50.0 57.9 58.0 2989-2992 bp 0.033898 24.9
ndhF_p2 TGAAAACAAAGAGAGACGTCCA CAGCGTGTCTACCGATTTCA 40.9 50.0 57.9 58.0 2990-2993 bp 0.033898 24.9
ndhF_p3 TGAAAACAAAGAGAGACGTCCA GAGTTCTGTCCCGGACTTTC 40.9 55.0 57.9 57.9 2740-2743 bp 0.033898 24.3
ndhF_p4 TGAAAACAAAGAGAGACGTCCA GGCGAGCTTGAAGGAGTTAA 40.9 50.0 57.9 57.9 2924-2927 bp 0.033898 24.2
ndhF_p5 TGAAAACAAAGAGAGACGTCCA TCCTAAGAGCAGCGTGTCTA 40.9 50.0 57.9 57.9 2999 bp 0.016949 22.6