Hotspot

ccsA-ndhD

SSC · 119806-120004 · 199 bp

Back to Salix

Pi 0.022
MarkerSeek score 56.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
199
variable_sites
17
pi
0.022
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CAATTGTTGCTTCGATCGGT CAGTTGACAAGGTCGAAGCT 45.0 50.0 57.1 58.1 346-369 bp 1.0 77.9
ccsA-ndhD_p2 CAATTGTTGCTTCGATCGGT TCAGTTGACAAGGTCGAAGC 45.0 50.0 57.1 58.1 347-370 bp 1.0 77.8
ccsA-ndhD_p3 TGTTGCTTCGATCGGTTTTC CAGTTGACAAGGTCGAAGCT 45.0 50.0 57.0 58.1 342-365 bp 1.0 77.5
ccsA-ndhD_p4 TGTTGCTTCGATCGGTTTTC TCAGTTGACAAGGTCGAAGC 45.0 50.0 57.0 58.1 343-366 bp 1.0 77.4
ccsA-ndhD_p5 CAATTGTTGCTTCGATCGGTT CAGTTGACAAGGTCGAAGCT 42.9 50.0 57.8 58.1 346-369 bp 1.0 75.5