Hotspot

ndhF-trnL-UAG

SSC · 118137-118654 · 518 bp

Back to Salix

Pi 0.0139
MarkerSeek score 53.30
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
518
variable_sites
35
pi
0.0139
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TCCATAAATATTGATATGTATGTTCCA AGCGTGTCTACCGATTTCAC 25.9 50.0 54.9 58.0 526-636 bp 1.0 48.8
ndhF-trnL-UAG_p2 TCCATAAATATTGATATGTATGTTCCA CAGCGTGTCTACCGATTTCA 25.9 50.0 54.9 58.0 527-637 bp 1.0 48.8
ndhF-trnL-UAG_p3 TCCATAAATATTGATATGTATGTTCCA TCCTAAGAGCAGCGTGTCTA 25.9 50.0 54.9 57.9 536-646 bp 1.0 48.7
ndhF-trnL-UAG_p4 TCCATAAATATTGATATGTATGTTCCA CCTAAGAGCAGCGTGTCTAC 25.9 55.0 54.9 57.8 535-645 bp 1.0 48.7
ndhF-trnL-UAG_p5 TCCATAAATATTGATATGTATGTTCCA CTAAGAGCAGCGTGTCTACC 25.9 55.0 54.9 57.8 534-644 bp 1.0 48.7