Hotspot

trnR-ACG-trnN-GUU

IRb · 109076-109801 · 726 bp

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Pi 0.00435
MarkerSeek score 55.90
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
726
variable_sites
22
pi
0.00435
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 AACAGATCCAGTGGAGACGA CCGTTGTTCCTCAGTAGCTC 50.0 55.0 58.1 58.0 752-1017 bp 1.0 85.3
trnR-ACG-trnN-GUU_p2 TAGGGTATTCCGGGGAACAG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 58.2 58.0 767-1032 bp 1.0 84.2
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 716-981 bp 1.0 84.2
trnR-ACG-trnN-GUU_p4 AACAGATCCAGTGGAGACGA GCTGTTAACTGACTGGTCGT 50.0 50.0 58.1 57.8 716-981 bp 1.0 84.2
trnR-ACG-trnN-GUU_p5 AACAGATCCAGTGGAGACGA TGGTCGTAGGTTCGAATCCT 50.0 50.0 58.1 58.2 703-968 bp 1.0 84.2