Hotspot

ndhC-trnV-UAC

LSC · 50047-51358 · 1312 bp

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Pi 0.00713
MarkerSeek score 47.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1312
variable_sites
56
pi
0.00713
unique_haplotype_count
30
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGCCCGAAATTAGAAATGCC GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.5 58.7 1010-1746 bp 1.0 77.8
ndhC-trnV-UAC_p2 GGCCCGAAATTAGAAATGCC TTCGAGTCCGTATAGCCCTA 50.0 50.0 57.5 57.1 1009-1745 bp 1.0 75.7
ndhC-trnV-UAC_p3 GGTCCTTTTCTAATTGGGGCT GTTCGAGTCCGTATAGCCCT 47.6 55.0 57.9 58.7 1035-1771 bp 1.0 73.0
ndhC-trnV-UAC_p4 TGGTCCTTTTCTAATTGGGGC GTTCGAGTCCGTATAGCCCT 47.6 55.0 58.2 58.7 1036-1772 bp 1.0 72.3
ndhC-trnV-UAC_p5 GCCCGAAATTAGAAATGCCA GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.8 58.7 1009-1745 bp 1.0 71.9