Hotspot

psbZ-trnG-GCC

LSC · 35675-36199 · 525 bp

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Pi 0.0125
MarkerSeek score 60.20
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
525
variable_sites
49
pi
0.0125
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CGGTTGGTCAAGCAACAAAA AATCGAACCCGCATCTTCTC 45.0 50.0 57.7 58.1 495-698 bp 1.0 87.1
psbZ-trnG-GCC_p2 CGGTTGGTCAAGCAACAAAA GAATCGAACCCGCATCTTCT 45.0 50.0 57.7 58.1 496-699 bp 1.0 87.1
psbZ-trnG-GCC_p3 TTTCTTCTCCTGACGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.5 58.1 508-711 bp 1.0 84.9
psbZ-trnG-GCC_p4 TTTCTTCTCCTGACGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.5 58.1 509-712 bp 1.0 84.9
psbZ-trnG-GCC_p5 TTCTTCTCCTGACGGTTGGT AATCGAACCCGCATCTTCTC 50.0 50.0 58.6 58.1 507-710 bp 1.0 84.6