Hotspot

psbM-trnD-GUC

LSC · 28623-29792 · 1170 bp

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Pi 0.00435
MarkerSeek score 54.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1170
variable_sites
32
pi
0.00435
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 1213-1288 bp 1.0 64.4
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC TTTCGTTTCCCTGGGATTGT 45.0 45.0 55.8 57.3 1235-1310 bp 1.0 62.8
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC CGTTTCCCTGGGATTGTAGT 45.0 50.0 55.8 57.2 1232-1307 bp 1.0 61.9
psbM-trnD-GUC_p4 AGGAACTAGAATGAACAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.4 58.5 1220-1295 bp 1.0 61.2
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.8 57.0 1214-1289 bp 1.0 60.2