Hotspot

rpoB-trnC-GCA

LSC · 25469-26669 · 1201 bp

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Pi 0.00847
MarkerSeek score 55.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1201
variable_sites
57
pi
0.00847
unique_haplotype_count
33
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCATTTCCATCCCCAAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 58.2 58.1 1256-1310 bp 1.0 85.3
rpoB-trnC-GCA_p2 CATTCCCCCATTTCCATCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.3 58.1 1263-1317 bp 1.0 84.6
rpoB-trnC-GCA_p3 CCATTTCCATCCCCAAGCAT AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 58.4 1238-1292 bp 1.0 83.1
rpoB-trnC-GCA_p4 ACATTCCCCCATTTCCATCC CCGGATTTGAACTGGGGAAA 50.0 50.0 57.5 58.1 1264-1318 bp 1.0 83.1
rpoB-trnC-GCA_p5 CATTCCCCCATTTCCATCCC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.3 58.4 1245-1299 bp 1.0 82.5