Hotspot

trnT-CGU-trnR-UCU

LSC · 7843-8075 · 233 bp

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Pi 0.0122
MarkerSeek score 54.10
Primer available yes
Label trnT-CGU-trnR-UCU

Feature Metadata

label_name
trnT-CGU-trnR-UCU
feature_type
igs
region
LSC
length_bp
233
variable_sites
24
pi
0.0122
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-CGU-trnR-UCU_p1 TTCCAAGCTAATGATGCGGG AGGTTTAGAAGACCTTTGTCCT 50.0 40.9 58.3 56.7 295-337 bp 1.0 61.5
trnT-CGU-trnR-UCU_p2 TTCCAAGCTAATGATGCGGG AGAAGACCTTTGTCCTATCCA 50.0 42.9 58.3 55.7 289-331 bp 0.983051 59.6
trnT-CGU-trnR-UCU_p3 TTCCAAGCTAATGATGCGGG TCTGGAAAAATATTGCGTCCA 50.0 38.1 58.3 55.9 338-380 bp 0.949153 58.5
trnT-CGU-trnR-UCU_p4 GCTAATGATGCGGGTTCGAT TCTGGAAAAATATTGCGTCCA 50.0 38.1 58.5 55.9 332-374 bp 0.949153 57.3
trnT-CGU-trnR-UCU_p5 TTCCAAGCTAATGATGCGGG TCTGGAAAAATATTGCGTCCAA 50.0 36.4 58.3 56.6 338-380 bp 0.949153 56.5