Hotspot

trnS-GCU-trnT-CGU

LSC · 6454-7072 · 619 bp

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Pi 0.00708
MarkerSeek score 53.80
Primer available yes
Label trnS-GCU-trnT-CGU

Feature Metadata

label_name
trnS-GCU-trnT-CGU
feature_type
igs
region
LSC
length_bp
619
variable_sites
32
pi
0.00708
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnT-CGU_p1 CAATCCGACGCTTTAGTCCA GCCGACGGATCCCTTAACTA 50.0 55.0 58.0 59.0 721-749 bp 1.0 79.7
trnS-GCU-trnT-CGU_p2 AATCCGACGCTTTAGTCCAC GCCGACGGATCCCTTAACTA 50.0 55.0 58.0 59.0 720-748 bp 1.0 79.7
trnS-GCU-trnT-CGU_p3 ATCAACGGAACCGGAAAGAG GCCGACGGATCCCTTAACTA 50.0 55.0 57.9 59.0 785-813 bp 1.0 78.7
trnS-GCU-trnT-CGU_p4 CATCAACGGAACCGGAAAGA GCCGACGGATCCCTTAACTA 50.0 55.0 58.2 59.0 786-814 bp 1.0 77.9
trnS-GCU-trnT-CGU_p5 TCATCAACGGAACCGGAAAG GCCGACGGATCCCTTAACTA 50.0 55.0 58.2 59.0 787-815 bp 1.0 77.9