Hotspot

psbI-trnS-GCU

LSC · 6217-6365 · 149 bp

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Pi 0.0207
MarkerSeek score 59.90
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
149
variable_sites
12
pi
0.0207
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 ATGATCCAGGGCGTAATCCT TGGACTAAAGCGTCGGATTG 50.0 50.0 58.3 58.0 251-272 bp 1.0 85.6
psbI-trnS-GCU_p2 ATGATCCAGGGCGTAATCCT GTGGACTAAAGCGTCGGATT 50.0 50.0 58.3 58.0 252-273 bp 1.0 85.6
psbI-trnS-GCU_p3 ATGATCCAGGGCGTAATCCT ATTGGGAGAGATGGCTGAGT 50.0 50.0 58.3 58.1 270-291 bp 1.0 84.3
psbI-trnS-GCU_p4 AATGATCCAGGGCGTAATCC TGGACTAAAGCGTCGGATTG 50.0 50.0 57.4 58.0 252-273 bp 1.0 83.1
psbI-trnS-GCU_p5 AATGATCCAGGGCGTAATCC GTGGACTAAAGCGTCGGATT 50.0 50.0 57.4 58.0 253-274 bp 1.0 83.1