Hotspot

psbK-psbI

LSC · 5649-6105 · 457 bp

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Pi 0.00716
MarkerSeek score 47.60
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
457
variable_sites
18
pi
0.00716
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 TGGCAAGCTGCTGTAAGTTT AGGATTACGCCCTGGATCAT 45.0 50.0 58.3 58.3 542-581 bp 1.0 82.2
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG AGGATTACGCCCTGGATCAT 50.0 50.0 57.9 58.3 574-584 bp 0.932203 79.9
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT AGGATTACGCCCTGGATCAT 45.0 50.0 58.3 58.3 572-582 bp 0.932203 77.9
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT AGGATTACGCCCTGGATCAT 45.0 50.0 58.3 58.3 573-583 bp 0.932203 77.9
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG GGATTACGCCCTGGATCATT 50.0 50.0 57.9 57.4 573-583 bp 0.932203 77.5