Hotspot

trnH-GUG-psbA

LSC · 76-296 · 221 bp

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Pi 0.011
MarkerSeek score 58.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
221
variable_sites
15
pi
0.011
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 TCACAATCCACTGCCTTGAG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.8 57.9 300-328 bp 1.0 85.3
trnH-GUG-psbA_p2 TCACAATCCACTGCCTTGAG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.8 57.9 303-331 bp 1.0 85.2
trnH-GUG-psbA_p3 GCGAATGACGGGAATTGAAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.8 57.9 335-363 bp 1.0 84.7
trnH-GUG-psbA_p4 GCGAATGACGGGAATTGAAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.8 57.9 338-366 bp 1.0 84.5
trnH-GUG-psbA_p5 GCGAATGACGGGAATTGAAC TAGACCTAGCTGCTGTCGAA 50.0 50.0 57.8 57.9 331-359 bp 1.0 84.3