Hotspot

ycf1

IRb · 107886-109235 · 1350 bp

Back to Salicornia

Pi 0.00796

MarkerSeek score 41.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1350
variable_sites: 23
pi: 0.00796
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	GGAGGGGGTCGAATTTCTTC	55.0	55.0	58.0	58.0	1746-2034 bp	1.0	82.2
ycf1_p2	GCTTCCTTCGTAACTCCAGG	GGAGGGGGTCGAATTTCTTC	55.0	55.0	58.0	58.0	1666-1954 bp	1.0	82.2
ycf1_p3	CGGGAGAATCAATGACTGCA	GGAGGGGGTCGAATTTCTTC	50.0	55.0	58.0	58.0	2079-2367 bp	1.0	81.6
ycf1_p4	CAATTCCCGTTCTCAACCCA	GGAGGGGGTCGAATTTCTTC	50.0	55.0	58.1	58.0	1705-1993 bp	1.0	81.5
ycf1_p5	AGCTAAAATGCGTTCCTCGT	GGAGGGGGTCGAATTTCTTC	45.0	55.0	57.9	58.0	2146-2434 bp	1.0	81.0