Hotspot

ndhF-rpl32

SSC · 111447-112540 · 1094 bp

Back to Salicornia

Pi 0.0204

MarkerSeek score 62.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1094
variable_sites: 49
pi: 0.0204
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAACTTGGAGTGGAAGGA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	57.9	57.9	1230-1271 bp	1.0	83.3
ndhF-rpl32_p2	GAAGTAAGCGGATCGAGCAA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	58.1	57.9	1454-1495 bp	1.0	83.0
ndhF-rpl32_p3	CGAAACTCGAAACTACCCGT	AAAAGGCTTTTAACGCTGCC	50.0	45.0	57.9	57.9	1790-1831 bp	1.0	82.5
ndhF-rpl32_p4	ACAAGCATTTGCAGCAATGG	AAAAGGCTTTTAACGCTGCC	45.0	45.0	58.2	57.9	1698-1739 bp	1.0	81.6
ndhF-rpl32_p5	AGAACCAGAATTGCAACGGT	AAAAGGCTTTTAACGCTGCC	45.0	45.0	58.0	57.9	1496-1514 bp	0.8	73.0