Hotspot

rpl20-rps12

LSC · 68615-69412 · 798 bp

Back to Salicornia

Pi 0.00927

MarkerSeek score 60.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 17
pi: 0.00927
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CCCTCGAAAGCTTGATCCAA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	883-903 bp	1.0	82.1
rpl20-rps12_p2	CCCTCGAAAGCTTGATCCAA	ACAGCCAATCCGAAATGTCA	50.0	45.0	57.9	57.8	935-955 bp	1.0	81.2
rpl20-rps12_p3	CCCTCGAAAGCTTGATCCAA	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	940-960 bp	1.0	80.6
rpl20-rps12_p4	CCCTCGAAAGCTTGATCCAA	CACAAGACAGCCAATCCGAA	50.0	50.0	57.9	58.5	941-961 bp	1.0	79.0
rpl20-rps12_p5	CCCTCGAAAGCTTGATCCAA	AGACAGCCAATCCGAAATGT	50.0	45.0	57.9	57.5	937-957 bp	1.0	78.8