Hotspot

rpl33-rps18

LSC · 67317-67692 · 376 bp

Back to Salicornia

Pi 0.0247

MarkerSeek score 61.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 21
pi: 0.0247
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CTAGTCGGTTGGAATTGCGA	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.0	58.0	1099-1232 bp	1.0	86.4
rpl33-rps18_p2	TTTTCCCAGATGCGTTGACA	CGTTTGTGGATCACTCGGAT	45.0	50.0	58.0	58.0	1493-1630 bp	1.0	85.9
rpl33-rps18_p3	CTAGTCGGTTGGAATTGCGA	GAACAAAACCGCAATCCGTT	50.0	45.0	58.0	57.9	703-840 bp	1.0	85.6
rpl33-rps18_p4	ATGCGTTGACATTCCCCTTT	CGTTTGTGGATCACTCGGAT	45.0	50.0	58.1	58.0	1484-1621 bp	1.0	85.5
rpl33-rps18_p5	AACCTATCTTTCCGTGGCAC	CGTTTGTGGATCACTCGGAT	50.0	50.0	57.9	58.0	1573-1710 bp	1.0	85.1